Human 20?-hydroxysteroid dehydrogenase (AKR1C1) inhibitor
     
Home > PRODUCTS > Biochemicals > Human 20?-hydroxysteroid dehydrogenase (AKR1C1) inhibitor

Academic Discounts 2018

Newsletter Subscription

Stay updated with our new products and services. You can unsubscribe at any time.


Human 20?-hydroxysteroid dehydrogenase (AKR1C1) inhibitor

3-Bromo-5-phenylsalicylic acid (NSC109116)
metabolism of progesterone in AKR1C1-overexpressed cells was potently inhibited by 3-bromo-5-phenylsalicylic acid, which was effective from 10 nM with an IC50 value equal to 460 nM

NSC109116
OTAVAchemicals Catalogue Number: 7070707133
CAS Registry Number: 4906-68-7
Purity: 95%+ (HPLC)

Ref.: El-Kabbani et al. Structure-Guided Design, Synthesis, and Evaluation of Salicylic Acid-Based Inhibitors Targeting a Selectivity Pocket in the Active Site of Human 20?-Hydroxysteroid Dehydrogenase (AKR1C1). Journal of Medicinal Chemistry (2009), 52, 3259-3264


Abstract: The first design, synthesis, and evaluation of human 20?-hydroxysteroid dehydrogenase (AKR1C1) inhibitors based on the recently published crystal structure of its ternary complex with inhibitor are reported. While the enzyme-inhibitor interactions observed in the crystal structure remain conserved with the newly designed inhibitors, the additional phenyl group of the most potent compound, 3-bromo-5-phenylsalicylic acid, targets a nonconserved hydrophobic pocket in the active site of AKR1C1 resulting in 21-fold improved potency (Ki = 4 nM) over the structurally similar 3?-hydroxysteroid dehydrogenase isoform (AKR1C2). The compound is hydrogen bonded to Tyr55, His117, and His222, and the phenyl ring forms additional van der Waals interactions with residues Leu308, Phe311, and the nonconserved Leu54 (Val in AKR1C2). Additionally, the metabolism of progesterone in AKR1C1-overexpressed cells was potently inhibited by 3-bromo-5-phenylsalicylic acid, which was effective from 10 nM with an IC50 value equal to 460 nM.

 
SSL